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. 2016 Feb 3;7(21):30258–30275. doi: 10.18632/oncotarget.7162

Figure 4. Impaired miRNA maturation in HTLV-1 infected cells lines.

Figure 4

A. Schematic representation of the experimental design to detect pre-miRNAs and mature miRNAs. MicroRNA genes are transcribed as large primary transcripts that are processed by a protein complex containing Drosha, to form an approximately 70 nucleotide hairpin precursor microRNA (pre-microRNA). This precursor is subsequently transported to the cytoplasm where it is processed by DICER, to form a mature microRNA of approximately 22 nucleotides. Pre-miRNAs were detected using forward primers that specifically hybridized with the pre-miRNA (but not the mature miRNA). In both case an reverse universal primer was used fo qPCR amplification. B-C. The levels of Let-7 pre-miRNAs (B) and mature miRNAs (C) were measured using qRT-PCR and normalized to U6 in two controls T-cell lines (CEM and Jurkat) and three HTLV-1 infected T-cells lines (Hut-102, C81-66 and ATL-2). Data are the means ± S.D. from three independent experiments (**p<0.01).