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. 2016 Feb 3;7(21):30258–30275. doi: 10.18632/oncotarget.7162

Figure 7. HBZ but not Tax decreases Dicer Expression.

Figure 7

A. Decreased RNA silencing in HTLV-1 infected cells expressing HBZ. HTLV-1 infected cells lines and control cell lines were transfected with a Renilla luciferase (RL)-miR-Let7 reporter plasmid. Activities of Renilla luciferase (RL)-miR-Let7 reporter in each cell line are expressed in relation to activities of RL-control reporter (set as 100%). Values are means ± S.D. of four transfections (**p<0.01; ***p<0.001). B-C. HBZ but not Tax decreases the efficiency of RNA interference mediated by the endogenous microRNA miR-let7 in 293T expressing transiently (B) or stably HBZ (C). Activities of Renilla luciferase (RL)-miR-Let7 reporter in each cell line are expressed in relation to activities of RL-control reporter (set as 100%). Values are means ± S.D. of four transfections (**p<0.01; ***p<0.001). D. 293T cells were transfected with a control plasmid (pcDNA) (lane 1), Tax plasmid (lane 2) or HBZ-Myc plasmid (lane 3). Forty-eight hours after transfection, Dicer, Drosha, HBZ, Tax and nucleolin expression were assessed by western blotting analysis. E. Dicer, Drosha, HBZ, Tax and nucleolin expression were assessed from lysates of two controls T-cell lines CEM and Jurkat (lanes 1 and 2) and HTLV-1 infected T-cells lines Hut-102, C81-66 and ATL-2 (lanes 3-5) by western blot. Dicer signals were quantified by densitometry and represented as a percentage of Dicer levels in the control group.