Figure 9. Valproate treatment of CD4+ T cells isolated from ATL patients restore Dicer expression.

A-C. Two controls T-cell lines (CEM; Jurkat) and three HTLV-1 infected T-cells lines (Hut-102; C81-66 and ATL-2) were treated with (black histogram) or without VPA (white histogram). Relative expression of Dicer, Tax and HBZ was measured by quantitative RT-PCR. Significant differences in expression are indicated by asterisk (**p<0.01; ***p<0.001). D-F. Relative expression of Dicer, Tax as well as HBZ was measured by quantitative RT-PCR in CD8+-cell–depleted PBMCs from HTLV-1 asymptomatic carriers (AC) and patients with acute ATL (ATL) treated (grey boxes) or not (white boxes) with VPA. Significance difference is indicated by asterisk (*p< 0.05; **p<0.01). G-H. AP-1-mediated activation of Dicer proximal promoter. 293T cells and Jurkat cells (G) were transiently transfected with the pGL3-prox Dicer-Luc reporter plasmid along with indicated expression vectors for Tax or JunD. Forty-eight hours later, luciferase activity was analyzed and normalized to β-Galactosidase activity. Data (mean and standard deviation) are representative of three independent experiments. (H) Forty-eight hours after transfection, Tax, JunD and actin expression were assessed by western blotting analysis. I-J. Summary heat map of differentially expressed miRNAs. Two controls T-cell lines CEM and Jurkat and the three HTLV-1 infected T-cells lines Hut-102, C81-66 and ATL-2 were treated with or without VPA. Heat map summarizing the patterns of expression for 13 miRNAs that were differentially expressed in HTLV-1 infected cell lines subtypes without VPA (I) and with VPA treatment (J).