Table 4. Primers used for RT-PCR.
| Gene Symbol | Gene Name | RefSeqRNA GenBank Accession No. | Primer pairs sequence (5′ → 3′)a | RT-PCR product size (base pair) |
|---|---|---|---|---|
| TSPAN8 | Tetraspanin 8 | NM_004616 | ctaagtctgatcgcattgtgaacacaattatggcttcctg | 103 |
| EPCAM | Epithelial cell adhesion molecule | NM_002354 | gtatgagaaggctgagataaagcttcaaagatgtcttcgtcc | 147 |
| SPINK1 | Serine peptidase inhibitor, Kazal type 1 | NM_003122 | tgaaaatcggaaacgccagacgcggtgacctgatgggattt | 89 |
| COL1A2 | Collagen, type I, alpha 2 | NM_000089 | gtggttactactggattgacctgccagcattgatagtttc | 184 |
| CDH1 | Homo sapiens cadherin 1, type 1, E-cadherin | NM_004360 | cagtacaacgacccaacccacacgctgacctctaaggtgg | 99 |
| LGALS4 | Lectin, galactoside-binding, soluble, 4 | NM_006149 | ttaccctggtcccggacattagcctcccgaaatatggcac | 135 |
| CEACAM6 | Carcinoembryonic antigen-related adhesion molecule 6 | NM_00248 | cacagtctctggaagtgctccggccagcactccaatcgt | 99 |
| B2M | Beta-2-microblobulin | NM_004048 | tgcctgccgtgtgaaccatgttgcggcatcttcaaacctccatga | 97 |
a
Top: forward primer; bottom: reverse primer (for each gene).