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. 2016 Apr 14;7(21):30336–30349. doi: 10.18632/oncotarget.8733

Figure 2. SENP7L regulates PC2-HP1α interaction and PC2 SUMOylation.

Figure 2

A. PC2, HP1α, and SENP7L interact at the chromatin. Chromatin-bound HP1α was isolated from MCF7 cells and interaction with PC2 and/or select SENP7 isoforms was evaluated with Western Blot analysis. B. SENP7L deSUMOylates PC2. In in vivo SUMOylation studies, modification of isolated chromatin-bound PC2 is identified with SUMO2/3 antibody in the presence and absence of SENP7L. C. SENP7L dictates PC2's SUMOylation and binding to HP1α. MCF7 cells were treated with the appropriate siRNA for 48hr and subsequently the modification state of the chromatin-bound PC2 was established as described above.