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. 2016 Mar 30;7(21):30420–30439. doi: 10.18632/oncotarget.8483

Figure 8. Curcumin modulates MARCKS expression in K562 cells and HUVECs.

Figure 8

(A) Real time PCR analysis showed that in K562 cells MARCKS mRNA expression decreases after treatment with Curcumin respect to untreated cells. Real time PCR analysis showed that MARCKS mRNA expression decreased in HUVECs treated with Curcu-exosomes respect to control exosomes. (B) Expression of MARCKS was evaluated in HUVECs transfected with 2-Ome-miR-21 (miR-21 inhibitor) treated or not with 20 μg/ml of control (miR-21 inhibitor + Exo 20 μg/ml) and Curcu-exosomes (miR-21 inhibitor + Curcu-Exo 20 μg/ml). Expression of MARCKS was also evaluated in HUVECs transfected with miR-21 mimic (miR-21 mimic) treated or not with 20 μg/ml of control exosomes (miR-21 mimic + Exo 20 μg/ml) and Curcu-exosomes (miR-21 mimic + Curcu-Exo 20 μg/ml). Values (FOI: fold of induction) are the mean ± SD of 3 independent experiments *p ≤ 0.05, **p ≤ 0.01. (C) Western blotting analyses of MARCKS in HUVECs treated with Curcu-exosomes and control exosomes. (D) Densitometric analysis of Western blotting against MARCKS. (E) Facs analysis of MARCKS expression.