Figure 5.

(A) Primary tumors from RT2 AB6F2 mice, with or without functional complement C5, were scored for presence or absence of CD68+ macrophages. *indicates p < 0.05 as determined by Fisher's exact test. Tumor samples from 17 mice were used for this analysis. (B) Representative immunohistochemistry of CD68 in C5null (C5⁰) and C5-encoding RT2 AB6F2 mice. When present, CD68-positive cells localized to the edge of tumors. (C) Tumor volumes were determined for RT2 AB6F2 mice with and without functional complement C5. Statistical significance was determined by non-parametric Mann-Whitney analysis, which revealed p < 0.05. A total of 182 mice were analyzed. (D) Angiogenic islets were counted in a set of 10 week-old RT2 AB6F2 mice encoding at least one functional complement C5 gene (C5+), or no functional complement C5 gene (C5⁰). Four mice of each genotype were analyzed.