Figure 6. Cld7 palmitoylation and EMT gene expression.

(A, C) Wt, kd and rescue ASML cells were stained with antibodies against EMT markers, EMT-related signaling molecules and transcription factors; the mean percent ± SD of stained cells is shown. Significant differences to ASML^wt cells are indicated by *. (B, D) Total cell lysates and (E) the nuclear fraction of the cells as above were separated by SDS-PAGE and blotted with the indicated antibodies; (F) Lysates were precipitated with anti-cld7 and blotted with anti-Oct3/4, -Snail, -Nanog and -Notch. A cld7^kd severely affects EMT signaling molecules and transcription factors, with the exception of Slug. Reduced recovery accounts particularly for the nuclear fraction. N-cadherin, FN and vimentin expression is reduced. These changes are not reverted in ASML-cld7^mPalm cells. Only an EpC^kd is associated with E-cadherin upregulation.