Figure 7. The linkage between palmitoylated cld7, vesicle formation and vesicle transporter proteins.

(A) Colocalization of cld7 (red) and EpC (red) with vesicle formation and vesicle transport associated proteins (green) in wt, EpC^kd, cld7^kd and cld7^mPalm ASML cells. Digital overlays of confocal microscopy images are shown (scale bar: 10 μm); (B) WB and coimmunoprecipitation of cld7 with vesicle formation and transport proteins in wt, kd and rescue ASML cells. Only palmitoylation-competent cld7 colocalizes and coimmunoprecipitates with molecules engaged in GEM-supported vesicle formation and vesicle transport. Instead, palmitoylation-competent cld7 does not colocalize with caveolin.