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. 2016 Apr 22;7(21):30678–30690. doi: 10.18632/oncotarget.8929

Figure 5. PPS1D1 binding and activity evaluation on panel of lung cancer cells.

Figure 5

(A) PS expression levels of lung cancer cell lines HCC4017, H460, HCC95, H1693, H1395 and normal HBEC30KT by binding with FITC-Annexin V. Lung cancer cells exhibited high PS levels while HBEC30KT has lower levels of PS (Error bars represent standard deviation). (B) Standard MTS cell viability data for the treatment of PPS1D1 and control PC462D1 on same lung cancer cells lines and HBEC30KT cells shown in (A). PPS1D1 at 20 μM caused strong cell cytotoxicity on cancer cells, but not on HBEC30KT. (C) Treatment of same lung cancer cells lines and HBEC30KT shown in (A) with Propidium iodide (PI) and Hoechst 33342 dyes. PI stained nuclei of all the cancer cell lines at 20 μM of PPS1D1, but not HBEC30KT cells. A known cell membrane damaging agent, BAC treatment caused PI stain on all the cells lines tested.