Figure 3. TMZ/TRAM-34 treatment increases apoptosis in murine and human malignant glioma cells.

GL261, U87MG and GBM18 cells were treated with vehicle (control, C), TMZ, TRAM-34 or both (T+T) for 96 h. Cell apoptosis was detected by flow cytometry; data represent the mean value of Annexin V positive plus Annexin V/PI positive cells expressed as percentage of total cells. *p<0.05 and ***p<0.001 vs C; ° p<0.05, °° p<0.01 and °°° p<0.001 vs TMZ n=3 by One-Way ANOVA, Student-Newman-Keuls post-test.