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. 2016 Apr 16;7(21):30781–30796. doi: 10.18632/oncotarget.8761

Figure 7. TRAM-34/TMZ treatment decreases CSC proliferation.

Figure 7

A. Representative morphology of GBM enriched CSC cultured as neurospheres. Magnification 10X. Scale bar 500 μm B. Time course of the effect of TRAM-34 (2.5 μM) on current evoked in GBM enriched CSC cells (n= 10) by repeated voltage ramps (from −130 mV to + 50 mV, holding potential −70 mV). Acute application of TRAM-34 revealed the functional expression of KCa3.1 channels. Typical current trace in response to repeated ramps is shown in the inset. C. CSC were treated for 4 days with TMZ, TRAM-34 or both (T+T) and analyzed for proliferation measured as [3H]-thymidine incorporation. Data are expressed as % of vehicle-treated cells (control, C); * p<0.05 vs C; ° p<0.05 vs TMZ n= 3, One-Way ANOVA, Student-Newman Keuls post-test.