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. 2016 Apr 27;7(21):30977–30989. doi: 10.18632/oncotarget.9050

Figure 3. PPMP induces multinucleation and apoptosis in human esophageal cancer cells.

Figure 3

(A) KYSE30 cells were treated with DMSO or PPMP (2 or 5 μM) for 24 h and then stained with anti-β-tubulin (green), anti-γ-tubulin (red), and DAPI (blue). Flat cells containing multiple nuclei are indicated (arrows). Scale bar indicates 20 μm (× 600). The percentage of multinucleated cells was quantified (*p < 0.05 vs. untreated). (B) KYSE510 cells and each of (C) 3 human esophageal cancer cell lines were incubated with 5 μM PPMP for 0, 24, or 72 h. Cells were collected and apoptosis was then detected using flow cytometry and Annexin V staining (*p < 0.05 vs. 0 h). (D) Three esophageal cancer cell lines were treated with 5 μM PPMP and harvested at 0, 6, 12, or 24 h, and then expression of apoptotic markers was detected by immunoblotting.