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. 2016 Apr 18;7(21):30990–31000. doi: 10.18632/oncotarget.8824

Figure 1. KLF4 enhanced human osteosarcoma cells clonogenicity and growth.

Figure 1

shRNA mediated silencing of KLF4 expression in human osteosarcoma cell lines. a. and e. Western blot detected the expression of KLF4 in MG63 and SaOS2 cells. GAPDH was detected as loading control. b–c. and f–g. Colony formation assay was used to measure the clonogenicity of MG63 and SaOS2 cells with or without knockdown KLF4. The numbers of control cells were set as 100%.(n=3, mean ± SD, t-test, n**P<0.01 vs. shRNA CTR). d. and h. Cells with or without knockdown KLF4 were cultured for the days as indicated and cell growth was evaluated by CKK8 assay. Results are representative of three independent experiments. *P<0.05, **p<0.01 and ***p<0.001 vs Ctr. i–j. and l–m. Colony formation assay was used to measure the clonogenicity of MG63 and SaOS2 cells with or without overexpression of KLF4. The numbers of control cells were set as 100% (n=3, mean ± SD, t-test, **P<0.01 vs. CTR). Western blot was used to detect the expression level of KLF4. k. and n. CKK8 assay was used to measure the cell viability of MG63 and SaOS2 with or without overexpression of KLF4.