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. 2016 Apr 18;7(21):30990–31000. doi: 10.18632/oncotarget.8824

Figure 4. KLF4 upregulated CRYAB expression in human osteosarcoma cells.

Figure 4

a–d. MG63 and SaOS2 cells with or without stable knockdown KLF4 were generated. The expression level of CRYAB and KLF4 were analyzed by western blot and q-RT-PCR. Results are representative of three independent experiments. *P<0.05, **p<0.01 and ***p<0.001 vs Ctr. e–h. MG63 and SaOS2 cells with or without stable overexpressing KLF4 were generated using lentivirus vector. The expression level of CRYAB and KLF4 were analyzed by western blot and q-RT-PCR. i–j. Increasing amounts of KLF4 were transfected into U2OS cells. The expression levels of CRYAB and KLF4 were measured by western blot and q-RT-PCR.