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. 2016 Apr 20;7(21):31053–31066. doi: 10.18632/oncotarget.8842

Figure 6. HG-induced EndMT is mediated by the translocation of MEF2A to the cytoplasm and interactions with p38 MAPK and smads in HUVECs.

Figure 6

A. and B. Immunofluorescence analysis of MEF2A and phosphorylated-MEF2A localization in HUVECs. MEF2A is stained green, nuclei are stained blue with DAPI (scale bar =3 μm); C. Western blot analysis of MEF2A and phosphorylated-MEF2A expression levels in cytoplasm and nuclear proteins of HUVECs; D. Western blot analysis of phosphorylated-p38, p38, phosphorylated-MEF2A, MEF2A, CD31, VE-Cadherin, FSP-1 and α-SMA in HUVECs after HG treatment with or without SB203580; E. CO-IP analysis of the protein-protein interaction between MEF2A and Smad2. F. PLA analysis of the protein-protein interaction between MEF2A and Smad2. The purple-stained dots represent MEF2A/Smad2-binding proteins. Nuclei are stained blue with DAPI (scale bar =3μm); G. Western blot analysis of MEF2A, phosphorylated-Smad2 and Smad2in HUVECs after HG treatment with or without MEF2A knockdown; H. Western blot analysis of Smad2, MEF2A, and EndMT markers in HUVECs after HG treatment with or without Smad2 knockdown by siRNA.