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. 2016 Apr 20;7(21):31067–31078. doi: 10.18632/oncotarget.8845

Figure 3. Re-expression of CYR61 rescues the inhibitory effect of CYR61 knockdown on cell migration.

Figure 3

A. The shCYR61 AGS cell line was infected by the lentiviral vector (FUW)-loaded CYR61 for 48 hours. The re-expressed CYR61 protein level was detected by immunoblotting. B. Migration of three different AGS cell lines was assessed by the transwell migration assay. C. For quantification, the dye crystal violet in the stained migrated cells was extracted with methanol and measured by absorbance at 540 nm. Data are from three independent experiments. EGF (50 ng/ml) was used for stimulation of cell migration. *** p<0.001.