Figure 7. Expression analyses in human breast cancer.
A and B. Tissues of breast cancer patients were divided into non cancer and cancer region, and H&E staining and immunohistochemistry using anti-ZNF224 antibody was carried out. C-E. Total RNAs were isolated from cancer and non-cancer regions of breast cancer patients, and the expression of ZNF224, miR-663a, and p53 was examined by qRT-PCR (n=14-18). GAPDH and U6 were used as loading control in ZNF224 and p53, and miR-663a, respectively.