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. 2016 Apr 29;7(21):31243–31256. doi: 10.18632/oncotarget.9111

Figure 6. 5-MTP inhibits TGF-β1-induced NF-κB activation in A549 cells.

Figure 6

(A) A549 cells were treated with 5-MTP for 30 min followed by TGF-β1 for 6 h. Cytosolic and nuclear fractions were prepared. p65 and p50 NF-κB in both fractions were analyzed with Western blotting. The Western blots are representative of two independent experiments with similar results. (B) A549 cells transfected with NF-κB promoter-luciferase reporter construct were treated with 5-MTP followed by TGF-β1 for 6 h. Promoter activity was analyzed as luciferase activity expressed as relative light unit (RLU). Bay11, a NF-κB inhibitor was included as positive control. Error bars denote mean ± SD (n = 3). * indicates P < 0.05.