Figure 7. p300 HAT is inhibited by 5-MTP but its inhibition does not influence EMT.

(A) A549 cells were treated with 5-MTP for 30 min followed by TGF-β1 for 24 h. p300 proteins were isolated by immunoprecipitation and HAT activity of IP-isolated p300 was measured. Error bars indicate mean ± SEM (n = 3). (B) A549 cells were pretreated with C646 for 30 min followed by TGF-β1 for 48 h. EMT markers were analyzed by Western blotting. Upper panels show representative Western blots and lower panels show densitometry. Error bars indicate mean ± SEM (n = 3). * indicates P < 0.05.