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. 2016 Apr 22;7(21):31413–31428. doi: 10.18632/oncotarget.8920

Figure 5. ART, DHA, and ARTS were partially dependent on Wnt/β-catenin signaling.

Figure 5

A. A549 cells were harvested, and Western blot analysis was conducted for β-catenin, Wnt5-a/b, NKD2, and Axin2 with or without IWP-2. B. Percentage of A549 cells in the G1 phase with 0 and 30 μM ART, DHA, and ARTS treatments accompanied with or without IWP-2. C. Cells were transfected with Wnt-5a siRNA or control siRNA before ART, DHA, and ARTS (30 μM) treatments. Wnt5a, β-catenin, sox2, and E-cadherin protein levels were detected by Western blot analysis. D. A549 cells were harvested, and Western blot analysis was performed for Axin2 and NKD2. E. H1299 cells were harvested, and Western blot analysis was conducted for Axin2 and NKD2. The data represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the control group (Con, control).