Figure 2. NEDL1 is not critical for mice survival.

A. Schematic diagram of the generation of Nedl1^−/− mice. Nedl1 mutant mice were generated from a C57BL/6J ES clone carrying the targeted Nedl1 allele designed to allow Cre-mediated deletion. Mice carrying the targeted Nedl1 allele were bred to EIIa-Cre transgenic mice to remove the neo^r gene and generate Nedl1^LacZ/+ reporter mice in which exon 8 are deleted and replaced with an IRES-LacZ gene. Offspring were intercrossed to generate homozygote Nedl1^LacZ/LacZ (Nedl1^−/−) mice. Exons are represented by blue boxes. B. Photograph of 18-month-old Nedl1^+/+ and Nedl1^−/− mice. C. ENS system detected by AChE-staining (upper panel) 2-month-old Nedl1^+/+ mice and Nedl1^−/− mice (Scale bar: 50 μm) and HE staining of kidneys (lower panel) from 6-month-old Nedl1^+/+ mice and Nedl1^−/− mice (Scale bar: 500 μm) show that there were no obvious defects in ENS and kidney. D. Analysis on HE staining of some tissue sections from 2-month-old Nedl1^+/+ mice and Nedl1^−/− mice. There were no obvious defects in brain, cerebellum, testis, heart, liver, spleen and lung. Scale bar: 50 μm.