Figure 3. AE suppresses the invasiveness of ovarian cancer cells in a dose-dependent manner.

SKOV3 cells were seeded onto the transwell insert consisting of 8 μm pore-size filters coated with Matrigel basement membrane matrix. Inserts were placed in wells containing 0, 100, 200, 300, 400 or 1000 μg/ml of AE in the medium and incubated for 24 h. A. Migrated cells were stained with crystal violet and photographed. Representative images (×200) show SKOV3 cells that crossed the matrigel coated membrane indicating invasiveness. B. Quantitative results of crystal violet released from migrated cells using spectrophotometry at 590 nm. Data are shown as Means ± SEM. from 4 independent observations. * indicates P<0.05 vs. the vehicle-treated control group. Bar=25 μM.