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. 2016 Apr 25;7(21):31484–31500. doi: 10.18632/oncotarget.8966

Figure 5. AE downregulates the expression of SNAIL1 and increases the expression of E-cadherin in SKOV3 cells.

Figure 5

SNAIL1 is a transfection factor that downregulates E-cadherin expression and promotes epithelial-mesenchymal transformation. SKOV3 cells were treated with AE (400 μg/ml) for 24 h and SNAIL1 and E-cadherin expression were studied. A, B. Immunocytochemical analysis shows that SNAIL1 positive cells (as percent of total cells) decreased after AE treatment. C, D. Western blot analysis shows that expression of SNAIL1 protein decreased in AE treated cells image and IGF1R/β-actin densitometry ratios. E, F. Immunocytochemical analysis shows increased number of E-cadherin positive cells (as percent of total number of cells) after AE treatment. G, H. Western blot analysis shows that E-cadherin protein expression increased in AE-treated cells. Representative blot image is shown along with bar graph showing IGF1R/β-actin densitometry ratios. Results are presented as Means±SEM *, P<0.05 vs. control, n=4 experiments. Insert bar=25 μM. β -actin was used as the loading control for Western blot analyses.