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. 2015 Dec 18;94(50):e2220. doi: 10.1097/MD.0000000000002220

FIGURE 2.

FIGURE 2

Genetic stability of ASCs in bone tumor context. Karyotype and FISH (FLI1-EWSR1 double fusion probe) analyses for the native tumor (A, arrow: t[11;22] [q24;q12]) and undifferentiated (B) and differentiated (C, arrow: t[14;15] [q32;q11.2]) ASC at P4 for patient 3: the FLI1-EWSR1 double fusion signals detected on the native tumour cells (A) were not detected in the ASCs at P4 (B, C), but minority tetrasomies of both probes by FISH suggesting tetraploidy were found for undifferentiated ASCs (B). Initial trisomy 8 was not detected, but minority tetraploidy (detected by the tetrasomies of both probes by FISH) was found after osteogenic differentiation (C) in comparison to the native tumor tissue. ASCs = adipose-derived stem cells, FISH = fluorescence in situ hybridization, P4 = passage 4.