Figure 1. Example of pathway activating construct activity validation.

Target cells (shown here, 293T) were infected with constructs encoding individual pathway activating constructs. Following selection with puromycin, whole cell lysates were collected and immunoblotted for the appropriate markers of pathway activation. For the MAPK pathway activator, constitutively active mutant of MEK1, phospho-ERK was used as an indicator of proper pathway activation.