Figure 6. TEAD1-Tg muscle exhibits faster regeneration than Wt muscle upon injury with BaCl2.
(A–Hi) H and E stains of Wt (A–Di) and TEAD1-Tg (E–Hi) TA muscle 3 days (B–Bi, F–Fi), 5 days (C–Ci, G–Gi), or 7 days (D–Di, H–Hi) after injury with BaCl2 or uninjured (A–Ai, E–Ei). Images Ai–Hi are higher magnification views of regenerating areas of muscle (regenerating fibers indicated by black arrows). I–J) Quantification of fiber area (I) and fiber diameter (J) for 5 days and 7 days after BaCl2 injury. K–R) IF images show myogenin (green) and DAPI (blue; K,M,O,Q) or embryonic myosin heavy chain (green) with laminin (red; L,N,P,R) localized to regenerating fibers in Wt (K–L, O–P) and TEAD1-Tg (M–N, Q–R) TAs 3 days and 5 days after BaCl2 injury. For quantification of fiber number and diameter n=3 mice were used. p<0.05 represented by (*), p<0.005 represented by (**), p<0.0005 represented by (***).