. Author manuscript; available in PMC: 2016 Oct 11.

Published in final edited form as: Mol Reprod Dev. 2010 Feb;77(2):136–148. doi: 10.1002/mrd.21114

TABLE 3.

Fresh Bovine Sperm Capacitated by Incubating With Ouabain or Anti-ATP1A4 Initiated Cleavage of Bovine Oocytes In Vitro

Group	No. of oocytes	Cleaved (%)
Negative	153	13.0 ± 1.1^a
Heparin	132	79.5 ± 4.0^b
Ouabain	141	74.4 ± 3.3^b
Anti-ATP1A4	167	53.8 ± 3.8^c
Pre-absorbed	157	19.1 ± 1.2^a
Pre-immune	149	12.0 ± 0.9^a

Sperm preparations were incubated with either Sp-TALP alone (negative), heparin (positive control for capacitation), ouabain, anti-ATP1A4, anti-ATP1A4 pre-absorbed to its blocking peptide or pre-immune serum for 4 hr. After incubation, sperm were washed in Sp-TALP and resuspended in final fertilization medium and used for inseminating in vitro-matured bovine oocytes.

Values without a common superscript (a–c) differed (P <0.05).