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. 2016 Sep;8(9):2580–2591. doi: 10.21037/jtd.2016.08.82

Table 1. Used antibodies and detection kits for immunohistochemical staining.

Antibody specificity Clone Company Dilution Detection kit
CD117: polyclonal rabbit anti-human c-kit Polyclonal Dako A/S 1:100 OptiViewKit
CD5: mouse anti-human CD5 4C7 Novocastra 1:20 OptiViewKit
p63: mouse anti-p63 4A4 Ventana Prediluted OptiViewKit
p40: mouse anti-p40 Polyclonal Zytomed Systems 1:50 OptiViewKit
p21 (C-19): rabbit-anti-p21 Polyclonal Santa Cruz Biotechnology, Inc. 1:50 ChromoMapKit
p27: rabbit anti-p27 Polyclonal Santa Cruz Biotechnology, Inc. 1:60 OptiViewKit
p53: mouse anti-human p53 protein DO-7 Dako A/S 1:80 OptiViewKit
Bcl-2: mouse anti-Bcl-2 124 Ventana Prediluted OptiViewKit
Ki-67: rabbit anti-Ki-67 30-9 Ventana Prediluted OptiViewKit
Podoplanin: mouse anti-human podoplanin D2-40 Dako A/S 1:50 OptiViewKit
Synaptophysin: mouse anti-synaptophysin 27G12 Novocastra 1:50 OptiViewKit
PTEN: rabbit anti-PTEN D4.3 Cell Signaling Technology 1:50 UltraViewKit
Pax8: rabbit anti-paired box 8 Polyclonal Protein Tech Group, Inc. 1:400 OptiViewKit