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. 2016 May 19;78(9):1405–1411. doi: 10.1292/jvms.16-0124

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©2016 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 1. — Electron microscopy and immunogold electron microscopy. (A) Swelling and deformity of viral structures are observed. Purified H5N3 viruses were pretreated with frHibis for 10 min. (B) Structures of avian influenza virus particles are intact. The purified viruses were pretreated with medium. (C) H5 HA antigen-positive viral particles are not observed. Purified H5N3 viruses pretreated with frHibis for 10 min were incubated with anti-H5 mAb followed by incubation with 10-nm gold-conjugated goat anti-mouse IgG + IgM. (D) H5 HA antigen-positive viral particles are observed. Untreated H5N3 viruses were incubated in the same manner.