Table 2. Species and genus specific primers used for real time PCR.

Gene	Primers (sense/antisense 5’-3’)	Size (bp)	Annealing temperature (°C)
TNF-α	F: CACCACGCTCTTCTGCCTACTG	115	63
	R: TTGAGACGATGATCTGAGTCCTTGG
MCP-1	F: GTCCTTGCCCAGCCAGATG	148	60
	R: CGATGGTCTTGAAGATCACTGCT
IL1-β	F: AAAGGGGACTTGAAGAGAG	286	58
	R: CTGCTTGAGAGGTGCTGATGT
IL-6	F: AAGGTGATGCCACCTCAGAC	151	60
	R: TCTGCCAGTACCTCCTTGCT
ZO-1	F: GGCGCACGGCGAAGGTAATT	405	60
	R: CTATCAAACTCAGGAGGCGGCACT
Occludin	F: GGAGTGATTCGGATTCTGTCTATGCT	423	60
	R: CGCCTGGGCTGTTGGGTTGA
β-actin	F: CCAGGTCATCACCATCGG	158	60
	R: CCGTGTTGGCGTAGAGGT