Table 1.
Sample of de novo synthesized proteins isolated from stably transfected HEK293 cells expressing hSloα after 6 h ethanol incubationa
| Protein | Gene _human | Accession number | Biological function | EtOH | Naive | Null | EtOH/naive |
|---|---|---|---|---|---|---|---|
| Ca2+activated K channel subunit α-1 | KCNMA1 | Q28204 | Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+ | 13 | 15 | 3 | 0.9 |
| Catenin β-1 | CTNB1 | P35222 | Key downstream component of the canonical Wnt signaling pathway | 14 | 7 | 0.5 | 2.0 |
| Spectrin β chain | SPTB2 | Q01082 | Involved in secretion, interacts with calmodulin in a calcium-dependent manner, and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane | 22 | 9 | 7 | 2.4 |
| Amyloid-like protein 2 | APLP2 | Q06481 | May play a role in the regulation of hemostasis; the soluble form may have inhibitory properties toward coagulation factors | 10 | 6 | 2 | 1.7 |
| Clathrin interactor 1 | EPN4 | Q14677 | Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2); may have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes; stimulates clathrin assembly | 9 | 3 | 1 | 3.0 |
| Junction plakoglobin | PLAK | P14923 | Common junctional plaque protein; can replace β-catenin in E-cadherin/catenin adhesion complexes, which are proposed to couple cadherins to the actin cytoskeleton | 32 | 21 | 14 | 1.5 |
| mRNA export factor | RAE1L | P78406 | Binds mRNA; may function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton | 6 | 4 | 0 | 1.5 |
| Coatomer subunit β | COPB | P53618 | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non–clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans-Golgi network | 12 | 5 | 1 | 2.4 |
| Polyadenylate-binding protein 1 | PABP1 | P11940 | Binds the poly(A) tail of mRNA | 35 | 28 | 9 | 1.3 |
| Clusterin | CLUS | P10909 | Extracellular chaperone that prevents aggregation of non-native proteins | 6 | 4 | 0 | 1.5 |
| Probable ATP-dependent RNA helicase DDX5 | DDX5 | P17844 | Involved in the alternative regulation of pre-mRNA splicing | 34 | 27 | 7 | 1.3 |
| BCL-6 corepressor | BCOR | Q6W2J9 | Transcriptional corepressor | 6 | 2 | 0 | 3.0 |
aSummed unique peptide count control (naive), 25 mM ethanol and null (no AHA control) all after 6 h incubation. Ratio of ethanol to naive highlights fold differences in peptide detection for each protein.