Skip to main content
. 2016 Oct 4;7:13015. doi: 10.1038/ncomms13015

Figure 1. Sequence alignment between N. meningitidis and N. gonorrhoeae PilE and other Type IV pilin-like proteins.

Figure 1

(a) Amino-acid sequence alignment between N. meningitidis (Nm) and N. gonorrhoeae (Ng) PilE, with secondary structures indicated above and below the sequences, respectively, based on the crystal structures of Nm ΔN-PilE (residues 29–161, this study) and full-length Ng PilE9,11. A grey bar represents α1N, which is absent in the Nm ΔN-PilE structure. The secondary structure of Nm PilE α1 within the T4P filament is indicated at the top of the panel based on the cryoEM density map. The amino-acid alignment was performed using CLUSTAL OMEGA69 with manual adjustments and visualized using ESPript70. Identical residues are shown in white font on a red background. Residues of interest are boxed and the conserved disulfide-bonded cysteines are connected. (b) N-terminal amino-acid sequence alignment among Type IV pilins from Gram negative and Gram positive bacteria, Type II secretion pseudopilins and archaeal flagellins. Conserved residues are boxed and/or highlighted. Glycines are also highlighted in the archaeal flagellins although their positions are not conserved. Nm PilE, N. meningitidis PilE (GenBank accession number WP_014573675), Ng PilE, Neisseria gonorrhoeae PilE (for 2HI2, P02974), Dn FimA, Dichelobacter nodosus (X52403), Pa PilA, Pseudomonas aeruginosa PilA (P02973); Gs, Geobacter sulfurreducens PilA (2M7G_A); Ft PilE1, Francisella tularensis Schu S4 PilE1 (CAG45522); Hi, non-typeable Haemophilus influenzae (AAX87353); Cd PilA1, Clostridium difficile PilA1 (ref. 71); Cp PilA2, Clostridium perfringens PilA2 (NP_563200); Ko PulG, Klebsiella oxytoca PulG (S11917); Vc EpsG, V. cholerae EpsG (AAA58788); Pa XcpT, P. aeruginosa XcpT (BAT65239); Ec BfpA, enteropathogenic E. coli BfpA (Z68186); Vc TcpA, V. cholerae TcpA (ABQ19609); Ec CofA, enterotoxigenic E. coli CofA (BAA07174); Ih Iho670, Igniococcus hospitalis Iho670 (WP_011998704); Hs FlgB2, Halobacterium salinarum FlgB2 (CAP13655); Ss FlaB, Sulfolobus sulfataricus FlaB (AKA77968); Mh Mhun, Methanospirillum hungatei Mhun_3140 (ABD42825).