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. 2016 Oct 4;7:12885. doi: 10.1038/ncomms12885

Figure 8. Correlative expressions of Wnt signalling modulators and miR-126 or miR-20b are prognostic for clinical GBM.

Figure 8

(a) Correlation of expression between miR-20b or miR-125b, and TCF4 or FZD6 in 61 snap-frozen clinical GBM tumour specimens. (b) Kaplan–Meier curves for survival of patients with GBM that have expression levels of miR-20b/miR-125b high and FZD6 low (upper), miR-20b/miR-125b high and TCF4 high (lower). (c) Representative images of IHC of TCF4 and FZD6 in a separate cohort of a total of 76 paraffin-embedded clinical GBM samples. Arrows, corresponding tumour cells in sister tumour sections that were stained positive or negative by indicated antibodies. Right, bar graphs indicate percentages of specimens showing high or low TCF4 expression relative to level of FZD6. Scale bar, 50 μm. (d) Kaplan–Meier analyses for survival of patients with GBM that have expression levels of TCF4 high/FZD6 low or TCF4 low/FZD6 high. (e) Percentages of specimens showing high or low miR-20b or miR-125b expression relative to levels of FZD6 in TCGA data sets. (f) Kaplan–Meier curves for survival of patients with GBM that have expression levels of miR-20b/miR-125b high and FZD6 low (left), and miR-20b/miR-125b high and TCF4 high (right) in TCGA data sets. In b,d and f, P values were calculated using log-rank and Gehan–Breslow–Wilcoxon tests. *P<0.05, **P<0.01, ***P<0.001, paired two-way Student's t-test. Data are representative from three independent experiments with similar results.