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. 2016 Sep 28;2016:3758941. doi: 10.1155/2016/3758941

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Copyright © 2016 Yi Ma et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of recombinant hEGF-SUMO-H₁₀ and hEGF-Mxe-SUMO-H₁₀. M: protein ladder marker shown in kDa on the left sides of panels, (a) SDS-PAGE analysis of the recombinant hEGF-SUMO-H₁₀ produced in BL21(DE3), lane 1: whole cell sample after induction for 4 h, lane 2: supernatant sample after induction for 4 h; (b) SDS-PAGE analysis of the recombinant hEGF-Mxe-SUMO-H₁₀ produced in BL21(DE3), lane 1: whole cell sample after induction for 4 h, lane 2: supernatant sample after induction for 4 h; (c) Western blot analysis of recombinant hEGF-Mxe-SUMO-H₁₀ produced in BL21(DE3), lane 1: supernatant sample after induction for 4 h.