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. 2016 Sep 28;2016:1289157. doi: 10.1155/2016/1289157

Table 2.

Relationships among the presence of icaA, icaD, icaB, icaC, fnbA, fnbB, clfA, cna, hla, and sarA genes, slime production, adherence capacity on MtPs, and hydrophobicity index in 63 different clinical MRSA associated with GAS pharyngitis infection.

Strain ID Biofilm phenotype on CRA Slime synthesis In vitro adherence (MtP) assay Hydrophobicity index ± SD Presence of adhesion genes
Adherence OD570 nm ± SD ∗Adherence ability icaA icaB icaC icaD sarA cna clfA fnbA fnbB hla
GSA-83 Black Producer 1.42 ± 0.213 ++ 26.3 ± 0.325 + + + + + + + + + +
GSA-22 Black Producer 1.79 ± 0.659 ++ 32.3 ± 0.336 + + + + + + + + + +
GSA-32 Strong black Producer 3.23 ± 0.986 +++ 29.4 ± 0.962 + + + + + + + + + +
GSA-A21 Black Producer 1.53 ± 0.286 ++ 40.3 ± 0.560 + + + + + + − + − −
GSA-127 Black Producer 0.91 ± 0.632 + 32.9 ± 0.123 − − − − + − − − + +
GSA-74 Strong black Producer 3.21 ± 0.215 +++ 41.3 ± 0.963 + + + + + + − − − −
GSA-45 Strong black Producer 3.09 ± 0.0236 +++ 44.2 ± 0.023 + + + + + + + + + +
GSA-150 Reddish black Nonproducer 0.19 ± 1.023 − 15.3 ± 0.965 − − − − + − + + + +
GSA-99 Reddish black Nonproducer 1.32 ± 0.963 ++ 16.4 ± 0.189 + + + + − + − − + +
GSA-103 Bordeaux red Nonproducer 0.47 ± 0.451 − 21.6 ± 0.651 + + + + + + + + + +
GSA-89 Strong black Producer 3.41 ± 0.238 +++ 29.3 ± 0.359 + + + + + + + + + +
GSA-50 Black Producer 3.06 ± 0.896 +++ 31.2 ± 0.158 + + + + + + + + + +
GSA-A8 Strong black Producer 2.53 ± 1.639 ++ 27.9 ± 0.958 + + + + + − + + + +
GSA-44 Strong black Producer 3.86 ± 0.127 +++ 24.6 ± 0.756 + + + + + + + − − −
GSA-46 Bordeaux red Nonproducer 0.39 ± 0.986 − 24.3 ± 0.286 + + + + + + − + + +
GSA-48 Black Producer 1.06 ± 1.028 + 20.9 ± 0.396 + + + + + + − + + +
GSA-54 Bordeaux red Nonproducer 0.49 ± 0.966 − 14.5 ± 0.362 − − − − + − + − + +
GSA-395 Strong black Producer 3.23 ± 0.523 +++ 29.5 ± 0.396 + + + + + + + + + +
GSA-68 Strong black Producer 3.51 ± 0.889 +++ 32.6 ± 0.325 + + + + + + + + + +
GSA-94 Bordeaux red Nonproducer 0.42 ± 0.365 − 18.6 ± 0.176 − − − − − − − − − −
GSA-104 Black Producer 1.48 ± 0.632 ++ 30.9 ± 0.963 − − − − − + + − + +
GSA-140 Strong black Producer 3.52 ± 0.023 +++ 36.2 ± 0.990 + + + + + + − + − −
GSA-145 Strong black Producer 3.22 ± 0.965 +++ 28.9 ± 1.230 + + + + + + − + − −
GSA-142 Reddish black Nonproducer 0.96 ± 1.036 + 30.6 ± 0.968 − − − − + − − − − −
GSA-70 Bordeaux red Nonproducer 0.23 ± 0.396 − 13.6 ± 0.869 − − − − − − − + + +
GSA-126 Strong black Producer 3.62 ± 0.325 +++ 43.6 ± 0.310 + + + + + + + + + +
GSA-A4 Black Producer 2.57 ± 0.635 ++ 21.5 ± 0.256 + + + + + + + + + +
GSA-92 Reddish black Nonproducer 0.39 ± 0.961 − 12.9 ± 0.178 + + + + + − − + + +
GSA-365 Black Producer 1.98 ± 0.362 ++ 36.5 ± 0.986 + + + + + − + + + −
GSA-A12 Black Producer 1.59 ± 0.589 ++ 21.9 ± 0.936 + + + + + + + + + +
GSA-A18 Bordeaux red Nonproducer 1.09 ± 0.698 + 19.2 ± 0.129 + + + + + − − + + −
GSA-297 Black Producer 1.96 ± 0.129 ++ 29.9 ± 0.326 + + + + + + + + + +
GSA-134 Reddish black Nonproducer 3.12 ± 0.396 +++ 13.6 ± 0.349 + + + + + + − + + −
GSA-75 Black Producer 1.79 ± 0.326 ++ 30.1 ± 0.559 + + + + + + + + + +
GSA-88 Strong black Nonproducer 3.09 ± 0.856 +++ 24.3 ± 0.552 + + + + + + + + + +
GSA-71 Black Producer 3.85 ± 0.785 +++ 22.3 ± 0.639 + + + + + + + + + +
GSA-A25 Black Producer 0.85 ± 0.759 + 26.8 ± 1.36 + + + + + − + + + +
GSA-79 Bordeaux red Nonproducer 0.21 ± 0.856 − 14.9 ± 0.759 − − − − − + − + + −
GSA-52 Black Producer 3.52 ± 0.996 +++ 23.6 ± 0.529 + + + + + + + − − +
GSA-91 Bordeaux red Nonproducer 0.86 ± 1.236 + 15.9 ± 0.169 + + + + + − + + + +
GSA-84 Strong black Producer 3.11 ± 1.036 +++ 40.1 ± 0.629 + + + + + + − + + +
GSA-53 Bordeaux red Nonproducer 0.36 ± 0.845 − 19.1 ± 0.785 + + + + + − + + + +
GSA-137 Black Producer 1.56 ± 0.965 ++ 22.6 ± 0.396 + + + + + + − + + +
GSA-A20 Strong black Producer 3.51 ± 0.515 +++ 42 ± 0.968 + + + + + + − + + +
GSA-291 Black Producer 1.63 ± 0.689 ++ 26.9 ± 0.236 + + + + + + − + + +
GSA-98 Black Producer 1.79 ± 0.632 ++ 22.6 ± 0.756 + + + + + + − + + +
GSA-73 Strong black Producer 3.24 ± 0.325 +++ 36.2 ± 0.688 + + + + + + − + + +
GSA-A16 Black Producer 2.06 ± 0.963 ++ 32.8 ± 0.895 + + + + − + + + + +
GSA-131 Bordeaux red Nonproducer 1.08 ± 0.896 + 16.3 ± 0.955 + + + + + − − + + −
GSA-410 Strong black Producer 3.69 ± 0.563 +++ 36.8 ± 0.269 + + + + + + + + + +
GSA-377 Bordeaux red Nonproducer 0.27 ± 1.342 − 17.2 ± 0.745 + + + + + + + + + −
GSA-A1 Black Producer 3.04 ± 0.506 +++ 21.1 ± 0.986 + + + + + + + − − +
GSA-A6 Bordeaux red Nonproducer 0.79 ± 0.966 + 13.9 ± 0.156 + + + + + + + + + −
GSA-A9 Strong black Nonproducer 2.89 ± 0.796 ++ 24.3 ± 0.969 + + + + + + + + + +
GSA-A17 Black Producer 1.85 ± 0.235 +++ 19.9 ± 0.589 + + + + + + + + + +
GSA-28 Bordeaux red Nonproducer 1.25 ± 0.168 + 19.2 ± 0.129 + + + + + + + − + −
GSA-51 Black Producer 2.57 ± 0.234 ++ 21.3 ± 0.345 + + + + + + − + + +
GSA-58 Reddish black Nonproducer 0.55 ± 0.996 + 18.2 ± 0.569 + + + + + + + + + −
GSA-63 Reddish black Nonproducer 1.43 ± 0.351 ++ 11.9 ± 0.266 − − − − − − − − − −
GSA-81 Reddish black Nonproducer 0.98 ± 0.029 + 19.1 ± 0.192 + + + + − − − − − +
GSA-86 Bordeaux red Nonproducer 0.49 ± 0.259 − 16.2 ± 0.367 − − − − − − + − − −
GSA-97 Strong black Nonproducer 3.39 ± 0.125 +++ 23.9 ± 0.121 + + + + + + + + + +
GSA-102 Black Producer 1.08 ± 0.985 + 24.5 ± 0.276 + + + + + + − + + −

∗: Indicating the varied adhering ability of isolates on polystyrene surface, where +++ represents highly adherent (OD570 values of >3.0), ++ represents strongly adherent (OD570 values of >2.0), + represents moderately adherent (OD570 values of >1.0–2.0) and − represents weakly adherent (OD570 values of >0.5–1.0).