Figure 5.

Effect of LPE on phosphorylation of rate-limiting or intermediate proteins in the FcεRI signaling cascade. IgE-sensitized RBL-2H3 cells were preincubated with LPE for 1 h and then stimulated with antigen for 10 min. The above cells were washed with 1x DPBS and lysed with cell lysis buffer. The expression of p-Lyn, p-Fyn, p-Syk, p-ERK, p-JNK, p-p38, p-Akt, p-PLCγ1, p-PLCγ2, p-PKCδ, or β-actin was determined as described in Section 2. Similar results were obtained in three independent experiments. ^∗∗ P < 0.01 versus DNP-HSA-treated group. (a) p-Syk, p-Fyn, and p-Lyn; (b) p-PLCγ1/2 and p-PKCδ; (c) p-JNK, p-ERK, p-p38, and p-Akt.