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IGF-1 and NAC increase GSH production and reduce MG132 induced ROS generation in SH-SY5Y cells. Cell cultures were treated with NAC or IGF-1, separately or together, as described for Figure 1, followed by MG132 (5 µM). (a) Glutathione assay results for the control and treated cells versus MG132 treated cells only. (b) Intracellular ROS levels. Level of significance is denoted as ∗ p ≤ 0.05 and ∗∗ p ≤ 0.005.
