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. 2016 Sep 28;2016:6564212. doi: 10.1155/2016/6564212

Figure 4.

Figure 4

IGF-1 and NAC reduce MG132 induced autophagy without changing amounts of ubiquitinated proteins. After each treatment (indicated in the figure), total cell lysates were prepared for Western blot against ubiquitin and LC3B (an autophagy marker) antibodies. (a) Western blot for ubiquitinated protein. (b) Western blot analysis for LC3. β-Actin served as the loading control. Level of significance is denoted as p ≤ 0.05 and ∗∗ p ≤ 0.005.