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The autophagy inhibitor 3-methyladenine (3-MA) attenuates MG132 induced PARP and caspase 3 cleavage. The cells were pretreated with 3-MA (1 mM) for 1 h and then treated with MG132 for 24 h. (a) Western blot analysis for PARP, cleaved PARP, and cleaved caspase 3. Densitometric analysis for cleaved PARP (b) and cleaved caspase (c) from three independent experiments. Level of significance is denoted as ∗ p ≤ 0.01.
