Figure 4. Relative expression of mt2, hsp70 and hsp90 in 96 hpf zebrafish larvae following exposure to 0 μg Cu/L (solid line) or 325 μg Cu/L (dashed line) at either 26.5 °C or 34 °C from <1 to 4 hpf.

Data are represented as mean ± S.E.M. (n = 6 pools, 30 embryos/pool). The expression is normalized to geometric mean of ef1a and actb. Different capital letters (A,B) indicate significant differences between temperature groups independent of Cu exposure. Asterisk (*) along a line indicates a significant effect of Cu exposure independent of temperature.