. 2016 Oct 12;6:34254. doi: 10.1038/srep34254

Table 3. List of specific primers used for quantitative real-time PCR.

Gene	Accession		Primer (5′–3′)	Size (bp)	E (%)	Ta	Reference
dnmt3a1	AB196917	F	GCTAAGTTTGGTAAAGTGCGG	103	96	58 °C	28
dnmt3a1	AB196917	R	GGATGTCCTCCTTATCATTCA	103	96	58 °C	28
dnmt3a2	AB196919	F	TAGGAAAGGCTTGTTTGAGGG	157	105	59 °C	28
dnmt3a2	AB196919	R	GCGTGAGATGTCTTTCTTGTC	157	105	59 °C	28
dnmt3b1	AB196915	F	GTGCCTCTGGGATGGATAAA	174	98	60 °C	Newly designed
dnmt3b1	AB196915	R	GCTCTGTGCACCACAGGATA	174	98	60 °C	Newly designed
dnmt3b2	AB196918	F	CGCTACATTGCCTCTGAGA	113	95	58 °C	28
dnmt3b2	AB196918	R	GCCAGATGTTTCCTAGTGATG	113	95	58 °C	28
dnmt3b3	AB196914	F	GCTCAGGTGCTGCTTTTTGTC	152	95	61 °C	28
dnmt3b3	AB196914	R	TTTTTGAATCTGTGCTTTGCTG	152	95	61 °C	28
dnmt3b4	AB196916	F	TCATTGACTTGGGGGAAGAG	172	97	60 °C	Newly designed
dnmt3b4	AB196916	R	TAATCATCATGGCTGCTGCT	172	97	60 °C	Newly designed
mt2	BC152694	F	AAGCTCTTTGTGGATACTCTCTGG	112	94	60.8 °C	Newly designed
mt2	BC152694	R	GCAGGTAGTACACTGGCAATTAGTG	112	94	60.8 °C	Newly designed
hsp70	AB062116	F	CATCGACGCCAACGGG	191	95	60 °C	61
hsp70	AB062116	R	CCAGGGAGTTTTTAGCAGAAATCTT	191	95	60 °C	61
hsp90	BC075757	F	GGTACCAAAGTCATTCTCCACCTT	154	97	61.8 °C	Newly designed
hsp90	BC075757	R	CCTCAAGATCCACCTCTTTTTCTC	154		61.8 °C	Newly designed
actb	BC165823	F	CCAACACTGTATTGTCTGGTGGTA	203	101	61 °C	Newly designed
actb	BC165823	R	GTACTCCTGCTTGCTAATCCACAT	203	101	61 °C	Newly designed
ef1a	AY422992	F	GGTACTACTCTTCTTGATGCCCTTG	200	98	60.8 °C	Newly designed
ef1a	AY422992	R	GACTTGACCTCAGTGGTTACATTG	200	98	60.8 °C	Newly designed

For each gene, its GenBank accession number, amplicon size (bp), amplification efficiency (E) and annealing temperature (Ta) are indicated.