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. 2016 Oct 12;6:35263. doi: 10.1038/srep35263

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Western blot analyses on P38, pP38, ERK, pERK, pJNK, pPI3K expressions in actein-treated HMEC-1 cells for 24 hours (or 48 hours for VEGFR1) were performed. (A) Immunoblotting was performed 3 times using independently prepared cell lysates and the representative blots were shown. (B) The histograms showed the quantified results of protein levels, which were adjusted with corresponding β-actin protein levels and expressed as fold of control (mean fold of control + SD from 3 independent experiments). Differences among the treated and vehicle-treated control groups were determined by one-way ANOVA with Tukey’s post-hoc test. *p < 0.05 as compared to control group.