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. 2016 Oct 12;6:35151. doi: 10.1038/srep35151

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Copyright © 2016, The Author(s)

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4^th instar larvae were injected with appropriate dsRNAs to induce gene silencing or with dsGFP in case of control. (a) RNAi of TcDad1 and double RNAi of TcStt3A and TcStt3B isoforms resulted in early larval mortality. (b) RNAi of TcGCS1 and TcGCS2α resulted in mortality during last larval and prepupal stages. Black arrow indicates dorsal split in the cuticle suggesting that pupation was initiated before the larvae died. (c) RNAi effects on larval growth (n = 51, 61, 43, 56, 58, 61 – top to down). (d) RNAi of TcStt3B but not of TcStt3A induced minor reduction of adult walking speed, n = 13. Error bars are SD, ***P < 0.001, **P < 0.01, *P < 0.05.