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. 2016 Oct 6;7:12977. doi: 10.1038/ncomms12977

Figure 1. LIrs1KO and LIrs2KO mice exhibited insulin resistance and glucose intolerance under a HF diet.

Figure 1

(a) Real-time RT–PCR analysis to determine the expression levels of Irs1 and Irs2 in liver biopsy specimens obtained from patients with NAFLD (n=30). (b) The expression levels of Irs1 and Irs2 in the livers obtained from mice on NC and HF diet (n=12–14). (c) Body weight gain in LIrs1KO and LIrs2KO mice on NC or a HF diet (n=9–20). (d–f) Insulin tolerance test (d), oral glucose tolerance test (e) and pyruvate tolerance test (f) in LIrs1KO and LIrs2KO mice on HF diet (n=13–20). (g) Expression levels of the gluconeogenic genes in the livers of LIrs1KO and LIrs2KO mice in the fasting and fed states (n=5–6). Results are represented as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, (Student's t-test was used to analyse the statistical significances of differences between two groups, and analysis of variance for the statistical significances of differences among multiple groups).