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. 2016 Oct 12;6:35316. doi: 10.1038/srep35316

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Copyright © 2016, The Author(s)

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(a,b) Representative photomicrograph and quantification of microtubule formation under hypoxia and normoxia. Differentiated cells under hypoxia had significantly enhanced vascular tube formation. Scale bars = 200 μm. (c) Expression of angiogenesis-associated factors (VCAM-1, SDF-1 and IGF-1) during EC differentiation under hypoxia and normoxia. Hypoxia generally improved these angiogenic factors. (d,e) Flow cytometry analysis was performed to compare the positive rate of induced ECs (CD34⁺CD31⁺KDR⁺) and each single endothelial marker between hypoxic and normoxic conditions. All data are represented as means ± SD. *P < 0.05 vs. normoxia group.