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. 2016 Oct 12;6:35099. doi: 10.1038/srep35099

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A,B) original micrographs of the MCTSs (U87MG cells) before image processing at the beginning (A, day 0) and at the end (B, day 8) of the invasion assay. (C,D) representation of the calculated MCTS features in overlay after image processing. The orange line depicts the core, the green line the halo and the blue color depicts the periphery of MCTS at day 1 (C) and day 8 (D). (E,F) results of the core extraction on the micrographs at day 0 (E) and day 8 (F). Representative micrographs of an MCTS are shown. Scale bar = 500 μm.