Figure 1. Inclusion level of exons 21+22 in CaV1.2 channels in neonatal hearts is higher than that in adult hearts.
(A) Exons 21 and 22 are mutually exclusive exons. RT-PCR across exons 19–25 could generate a fragment of 640 bp. Digestion with AvrII could produce two smaller fragments of 275 bp and 365 bp. Besides, aberrant exclusion or inclusion of both exons 21 and 22 would generate two fragments of 580 bp and 700 bp, respectively. (B) mRNA of CaV1.2 channels from both neonatal (NH) and adult (AH) hearts showed partial digestion by AvrII. Complete digestion was observed in control DNA containing exon 22. (C) Colony screening of a neonatal heart identified bands of three size classes: 580 bp, 640 bp and 700 bp. (D) Summary of aberrant splicing rate in rat neonatal hearts (NH, n = 5) and adult hearts (AH, n = 5). Data were shown as mean ± SEM. *p < 0.05.