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. 2016 Oct 12;6:35016. doi: 10.1038/srep35016

Figure 2. TRPM2 was required for HG-induced caspase-1-dependent IL-1β secretion in U937 monocytes.

Figure 2

(A,C) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. (A) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). (B,D) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of (B) GAPDH-, NLRP3-, ASC- or caspase-1-siRNA, or (D) GAPDH- or TRPM2-siRNAs under HG. (C) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. (A,C) *P < 0.05, **P < 0.01 and ***P < 0.001 vs. LG; #P < 0.05 and ##P < 0.01 vs. HG.