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. 2016 Oct 12;6:35016. doi: 10.1038/srep35016

Figure 4. TRPM2 knockdwon markedly reduced p47 phox phosphorylation, TXNIP expression and Trx activity under HG in U937 monocytes.

Figure 4

(A) Representative immunoblots and graphs for protein expressions of p47 phox, p22phox, gp91pox, TXNIP or GAPDH in the presence of BAPTA-AM (2.5, 5, 10 μM), or EGTA (0.5, 1, 5 mM) under low glucose (LG; 5.5 mM glucose) or high glucose (HG; 30 mM glucose) (n = 4–5). (B) Representative immunoblots and graphs for protein expressions of p47 phox, TXNIP or GAPDH or β-actin in the presence of GAPDH- or TRPM2-siRNA under HG (n = 4). (C) Quantitative PCR was performed on TXNIP mRNA in GAPDH- (GA si) or TRPM2-siRNA-treated cells under LG or HG, and it was normalized to LG + GAPDH-siRNA (n = 4). (D) TRX activity was determined by the insulin disulfide reduction assay, and was normalized to LG + GAPDH-siRNA. The cells were stimulated with HG for 24, 48, 72 h in the presence of GAPDH- or TRPM2-siRNA (n = 4). Data were shown as mean ± S.E.M. (C,D) *P < 0.05 and ***P < 0.001 vs. LG + GAPDH-siRNA; #P < 0.05 and ###P < 0.001 vs. HG + GADPH-siRNA.